Part:BBa_K103014:Design

OmpA_linker_alpha_linker

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1042
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Preparation of BBa_K103014 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20(BBa_K103014) here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).

BBa_K103006 was digested with EcoRI and SacI and ligated into pSB2K3 standard BioBrick vector (pSB2K3 with SacI site obtained by EcoRI/SacI digest of standard BioBrick vector pSB2K3 carrying BBa_K103017 part)